What does DNase I primarily target for digestion?

Unlock all questions

This demo includes only 20 questions. Upgrade to access hundreds of questions, flashcards, exam simulations, and disable ads.

Full question bankExam simulationsFlashcards
From $25.99Unlock all

Prepare for the AAB Molecular Diagnostics Test with focused study materials and practice questions. Gain insights into questions, formats, and key topics to excel in your exam and advance your career in molecular diagnostics.

Multiple Choice

What does DNase I primarily target for digestion?

Explanation:
DNase I is a nonspecific endonuclease that cleaves DNA by hydrolyzing phosphodiester bonds, and it works best on double-stranded DNA. It binds to the duplex and introduces nicks in the minor groove, cutting the two strands of the double helix. It can act on single-stranded DNA, but much less efficiently, and it doesn’t have specificity for particular bases or RNA. So the primary substrate it digests is double-stranded DNA.

DNase I is a nonspecific endonuclease that cleaves DNA by hydrolyzing phosphodiester bonds, and it works best on double-stranded DNA. It binds to the duplex and introduces nicks in the minor groove, cutting the two strands of the double helix. It can act on single-stranded DNA, but much less efficiently, and it doesn’t have specificity for particular bases or RNA. So the primary substrate it digests is double-stranded DNA.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy